Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema - Université Toulouse III - Paul Sabatier - Toulouse INP Accéder directement au contenu
Article Dans Une Revue EMBO Molecular Medicine Année : 2024

Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema

Regis Gayon
  • Fonction : Auteur

Résumé

Secondary lymphedema (LD) corresponds to a severe lymphatic dysfunction leading to the accumulation of fluid and fibrotic adipose tissue in a limb. Here, we identified apelin (APLN) as a powerful molecule for regenerating lymphatic function in LD. We identified the loss of APLN expression in the lymphedematous arm compared to the normal arm in patients. The role of APLN in LD was confirmed in APLN knockout mice, in which LD is increased and associated with fibrosis and dermal backflow. This was reversed by intradermal injection of APLN-lentivectors. Mechanistically, APLN stimulates lymphatic endothelial cell gene expression and induces the binding of E2F8 transcription factor to the promoter of CCBE1 that controls VEGF-C processing. In addition, APLN induces Akt and eNOS pathways to stimulate lymphatic collector pumping. Our results show that APLN represents a novel partner for VEGF-C to restore lymphatic function in both initial and collecting vessels. As LD appears after cancer treatment, we validated the APLN-VEGF-C combination using a novel class of nonintegrative RNA delivery LentiFlash vector that will be evaluated for phase I/IIa clinical trial.

Dates et versions

hal-04556533 , version 1 (23-04-2024)

Identifiants

Citer

Justine Creff, Asalaa Lamaa, Emeline Benuzzi, Elisa Balzan, Francoise Pujol, et al.. Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema. EMBO Molecular Medicine, 2024, 16 (2), pp.386-415. ⟨10.1038/s44321-023-00017-7⟩. ⟨hal-04556533⟩
13 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More