Article Dans Une Revue Science Année : 2023

Plant cell wall patterning and expansion mediated by protein-peptide-polysaccharide interaction

Gorka Fernandez
Ueli Grossniklaus

Résumé

Assembly of cell wall polysaccharides into specific patterns is required for plant growth. A complex of RAPID ALKALINIZATION FACTOR 4 (RALF4) and its cell wall–anchored LEUCINE-RICH REPEAT EXTENSIN 8 (LRX8)–interacting protein is crucial for cell wall integrity during pollen tube growth, but its molecular connection with the cell wall is unknown. Here, we show that LRX8-RALF4 complexes adopt a heterotetrametric configuration in vivo, displaying a dendritic distribution. The LRX8-RALF4 complex specifically interacts with demethylesterified pectins in a charge-dependent manner through RALF4’s polycationic surface. The LRX8-RALF4-pectin interaction exerts a condensing effect, patterning the cell wall’s polymers into a reticulated network essential for wall integrity and expansion. Our work uncovers a dual structural and signaling role for RALF4 in pollen tube growth and in the assembly of complex extracellular polymers.

Dates et versions

hal-04309257 , version 1 (27-11-2023)

Identifiants

Citer

Steven Moussu, Hyun Kyung Lee, Kalina Haas, Caroline Broyart, Ursina Rathgeb, et al.. Plant cell wall patterning and expansion mediated by protein-peptide-polysaccharide interaction. Science, 2023, 382 (6671), pp.719-725. ⟨10.1126/science.adi4720⟩. ⟨hal-04309257⟩
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