Cytotoxic, apoptotic, and sensitization properties of ent -kaurane-type diterpenoids from Croton tonkinensis Gagnep on human liver cancer HepG2 and Hep3b cell lines - Université Toulouse III - Paul Sabatier - Toulouse INP Accéder directement au contenu
Article Dans Une Revue Fundamental & Clinical Pharmacology Année : 2016

Cytotoxic, apoptotic, and sensitization properties of ent -kaurane-type diterpenoids from Croton tonkinensis Gagnep on human liver cancer HepG2 and Hep3b cell lines

Résumé

Human hepatocellular carcinoma (HCC) is the most common type of liver cancer, the second most common cause of death from cancer worldwide. A very poor prognosis and a lack of effective treatments make liver cancer a major public health problem, notably in less developed regions, particularly in eastern Asia. This fully justifies the search of new molecules and therapeutic strategies against HCC. Ent‐kaurane diterpenoids are natural compounds displaying a broad spectrum of potential therapeutic effects including anticancer activity. In this study, we analyzed the pharmacological properties of a family of ent‐kaurane diterpenoids from Croton tonkinensis Gagnep in human HepG2 and Hep3b cell lines, used as cellular reference models for in vitro evaluation of new molecules active on HCC. A structure‐related cytotoxicity was observed against both HCC cell lines, enlighting the role of the 16‐en‐15‐one skeleton of ent‐kaurane diterpenoids. Cytotoxicity was closely correlated to apoptosis, evidenced by concentration‐dependent subG1 cell accumulation, and increased annexin V expression. In addition, subtoxic concentration of ent‐kaurane diterpenoid dramatically enhanced the sensitivity of HCC cells to doxorubicin. All together, our data bring strong support to the potential interest of ent‐kaurane diterpenoids, alone or in combination with a cytotoxic agent, in cancer and more precisely against HCC.

Dates et versions

hal-03209715 , version 1 (27-04-2021)

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Minh Quan Pham, Anne Laure Iscache, Quoc Long Pham, Jean Edouard Gairin. Cytotoxic, apoptotic, and sensitization properties of ent -kaurane-type diterpenoids from Croton tonkinensis Gagnep on human liver cancer HepG2 and Hep3b cell lines. Fundamental & Clinical Pharmacology, 2016, 30 (2), pp.137-146. ⟨10.1111/fcp.12176⟩. ⟨hal-03209715⟩
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