Seeds of Peganum Harmala L. Chemical Analysis, Antimalarial and Antioxidant Activities, and Cytotoxicity Against Human Breast Cancer Cells
Résumé
The present study evaluated the levels of total phenolics, flavonoids, tannins and anthocyanins from Peganum harmala L. seeds and determined their antioxidant, antiplasmodial and anticancer potentials. Antioxidant activity was determined by DPPH and ABTS assays. Extracts of P. harmala seeds from Oudref and Djerba (two places in Tunisia) were obtained by successive extraction solvents: petroleum ether, chloroform, ethyl acetate, ethanol and water. Their composition was evaluated for phenolics (gallic acid equivalent 2.48 to 72.52 g/kg), tannins (catechin equivalent 0 to 25.27 g/kg), anthocyanins (cyanidin equivalent 0 to 20.56 mg/kg) and flavonoids (quercetin equivalent 0 to 3.12 g/kg). Ethanolic extract exerted the highest activities against a chloroquine-resistant strain of Plasmodium falciparum (IC₅₀=23 mg/L), against human breast cancer cells MCF7 (IC₅₀=32 mg/L) and against free radical (IC₅₀=19.09±3.07 mg/L). Correlations were studied between each chemical family and the three activities. Total phenolics content exhibited the highest correlation with antiplasmodial activity (R²=0.92) and with anticancer activity (R²=0.86), respectively.
Mots clés
Plasmodium falciparum / drug effects
Plasmodium falciparum / growth & development
Seeds / chemistry
Structure-Activity Relationship
Sulfonic Acids / antagonists & inhibitors
Tannins / isolation & purification
Anthocyanins / isolation & purification
Antimalarials / chemistry
Antimalarials / isolation & purification
Antimalarials / pharmacology
Antineoplastic Agents
Phytogenic / chemistry
Phytogenic / isolation & purification
Phytogenic / pharmacology
Antioxidants / chemistry
Antioxidants / isolation & purification
Antioxidants / pharmacology
Benzothiazoles / antagonists & inhibitors
Biphenyl Compounds / antagonists & inhibitors
Cell Survival / drug effects
Erythrocytes / drug effects
Erythrocytes / parasitology
Female
Flavonoids / isolation & purification
Humans
Liquid-Liquid Extraction
MCF-7 Cells
Parasitic Sensitivity Tests
Peganum / chemistry
Phenols / isolation & purification
Picrates / antagonists & inhibitors
Plant Extracts / chemistry